3AAW
Crystal structure of aspartate kinase from Corynebacterium glutamicum in complex with lysine and threonine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-6A |
Synchrotron site | Photon Factory |
Beamline | BL-6A |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2007-10-14 |
Detector | ADSC QUANTUM 4r |
Wavelength(s) | 0.978 |
Spacegroup name | I 4 |
Unit cell lengths | 162.232, 162.232, 133.926 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.560 - 2.500 |
R-factor | 0.21188 |
Rwork | 0.210 |
R-free | 0.25029 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB ENTRIES 2HMF and 2DTJ |
RMSD bond length | 0.008 |
RMSD bond angle | 1.066 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.590 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.056 | |
Number of reflections | 59981 | |
<I/σ(I)> | 27.1 | 5.8 |
Completeness [%] | 99.8 | 100 |
Redundancy | 7.6 | 7.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 1.2M Sodium citrate, 0.1M Tris-HCl, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |