3AAF

Structure of WRN RQC domain bound to double-stranded DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	90
Detector technology	CCD
Collection date	2007-09-30
Detector	Bruker DIP-6040
Wavelength(s)	0.9000
Spacegroup name	P 43 2 2
Unit cell lengths	84.077, 84.077, 145.291
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 1.900
R-factor	0.21339
Rwork	0.212
R-free	0.24354
Structure solution method	MAD
RMSD bond length	0.009
RMSD bond angle	1.409
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHARP
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.970
High resolution limit [Å]	1.900	1.900
Rmerge	0.043	0.488
Number of reflections	41719
<I/σ(I)>	14.7	6.2
Completeness [%]	100.0	99.9
Redundancy	9.7	9.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	9	293	20% PEG 4000, 0.2M sodium acetate, 0.1M CAPS-NaOH, pH 9, VAPOR DIFFUSION, temperature 293K
1	VAPOR DIFFUSION	9	293	20% PEG 4000, 0.2M sodium acetate, 0.1M CAPS-NaOH, pH 9, VAPOR DIFFUSION, temperature 293K
2