3A9H

Crystal Structure of PQQ-dependent sugar dehydrogenase holo-form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	CCD
Collection date	2009-02-27
Detector	ADSC QUANTUM 270
Wavelength(s)	1.0
Spacegroup name	I 41 2 2
Unit cell lengths	177.344, 177.344, 89.743
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.340 - 2.500
R-factor	0.20225
Rwork	0.201
R-free	0.23130
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3a9g
RMSD bond length	0.013
RMSD bond angle	1.592
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	CNS (1.2)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.500
Number of reflections	24995
Completeness [%]	99.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	sitting drop	6.6	293	ammonium sulfate, pH 6.6, sitting drop, temperature 293K