3A9G

Crystal Structure of PQQ-dependent sugar dehydrogenase apo-form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	CCD
Collection date	2009-02-27
Detector	ADSC QUANTUM 270
Wavelength(s)	1.0
Spacegroup name	I 41 2 2
Unit cell lengths	177.740, 177.740, 89.937
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.130 - 2.390
R-factor	0.20167
Rwork	0.201
R-free	0.21989
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ism
RMSD bond length	0.012
RMSD bond angle	1.519
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	CNS (1.2)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.390
Number of reflections	28570
Completeness [%]	99.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	sitting drop	6.6	293	ammonium sulfate, pH 6.6, sitting drop, temperature 293K