3A7Q
Structural basis for specific recognition of reelin by its receptors
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-10-03 |
| Detector | Bruker DIP-6040 |
| Wavelength(s) | 0.90 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 56.980, 93.844, 73.456 |
| Unit cell angles | 90.00, 107.41, 90.00 |
Refinement procedure
| Resolution | 46.930 - 2.600 |
| R-factor | 0.22695 |
| Rwork | 0.223 |
| R-free | 0.29603 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2e26 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.229 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.930 | 2.740 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.074 | 0.374 |
| Number of reflections | 21990 | |
| <I/σ(I)> | 7.5 | 1.8 |
| Completeness [%] | 96.6 | 97.5 |
| Redundancy | 3.7 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 36-38% MPD, 21-25% PEG 1000, 100mM HEPES-Na pH7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
