3A7N
Crystal structure of uracil-DNA glycosylase from Mycobacterium tuberculosis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-08-10 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 44.840, 63.670, 86.400 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 26.240 - 1.950 |
| R-factor | 0.18195 |
| Rwork | 0.181 |
| R-free | 0.19888 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2zhx |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.139 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.060 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Number of reflections | 18238 | |
| <I/σ(I)> | 13.7 | 3.2 |
| Completeness [%] | 98.2 | 97.7 |
| Redundancy | 4.8 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 7 | 298 | 1.8M tri-ammonium citrate pH 7.0, 10% isopropanol, microbatch, temperature 298K |
