Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B2 |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2007-11-20 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.282037 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 143.144, 146.767, 121.123 |
| Unit cell angles | 90.00, 109.24, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.500 |
| Rwork | 0.239 |
| R-free | 0.28200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2dkf |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | CNS |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 5.380 | 2.500 |
| Rmerge | 0.055 | 0.027 | 0.400 |
| Number of reflections | 81538 | ||
| <I/σ(I)> | 25.221 | ||
| Completeness [%] | 99.9 | 99.3 | 99.8 |
| Redundancy | 5.4 | 5 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | 50mM sodium citrate, 0.2M ammonium sulfate, 0.6M lithium sulfate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
