3A4T
Crystal structure of aTrm4 from M.jannaschii with sinefungin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2008-04-14 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.00 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 170.259, 40.097, 85.951 |
| Unit cell angles | 90.00, 110.84, 90.00 |
Refinement procedure
| Resolution | 42.450 - 2.300 |
| R-factor | 0.18551 |
| Rwork | 0.181 |
| R-free | 0.26756 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3A1X |
| RMSD bond length | 0.027 |
| RMSD bond angle | 2.284 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.450 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.022 | 0.564 |
| Number of reflections | 24668 | |
| <I/σ(I)> | 19.5 | 0.488 |
| Completeness [%] | 1.0 | 100 |
| Redundancy | 3.6 | 2.04 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 293 | 100mM Tris-HCl buffer (pH 7.4) , 200mM MgCl2 ,25% PEG 3350, 10mM cytidine, 3mM sinefungin, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
