3A2E

Crystal structure of ginkbilobin-2, the novel antifungal protein from Ginkgo biloba seeds

Replaces:  2E79

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	77
Detector technology	CCD
Collection date	2006-10-28
Detector	ADSC QUANTUM 210
Wavelength(s)	1.00000
Spacegroup name	P 21 3
Unit cell lengths	143.169, 143.169, 143.169
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.170 - 2.380
R-factor	0.208
Rwork	0.207
R-free	0.23200
Structure solution method	MAD
RMSD bond length	0.006
RMSD bond angle	1.001
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHELXS
Refinement software	PHENIX ((phenix.refine))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.470
High resolution limit [Å]	2.380	2.380
Total number of observations	602150
Number of reflections	39529
<I/σ(I)>	61.2	8.2
Completeness [%]	99.7
Redundancy	15.2	14.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.6	293	21% polyacrylic acid 5100, 100 mM MES, 20 mM MgCl2, pH 6.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K
1	VAPOR DIFFUSION, SITTING DROP	6.6	293	21% polyacrylic acid 5100, 100 mM MES, 20 mM MgCl2, pH 6.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K