3A1A
Crystal Structure of the DNMT3A ADD domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-10-19 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 57.581, 57.581, 138.949 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 28.790 - 2.300 |
R-factor | 0.21113 |
Rwork | 0.209 |
R-free | 0.24968 |
Structure solution method | MAD |
RMSD bond length | 0.012 |
RMSD bond angle | 1.316 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.796 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.063 | 0.410 |
Number of reflections | 6551 | |
<I/σ(I)> | 16.4 | |
Completeness [%] | 98.9 | 99.2 |
Redundancy | 12.3 | 12.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 17% PEG 8000, 0.1M Tris-HCl, 0.2M magnesium sulfate, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 17% PEG 8000, 0.1M Tris-HCl, 0.2M magnesium sulfate, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 17% PEG 8000, 0.1M Tris-HCl, 0.2M magnesium sulfate, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |