3ZY5
Crystal structure of POFUT1 in complex with GDP-fucose (crystal-form-I)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER |
| Synchrotron site | ESRF |
| Beamline | BM16 |
| Temperature [K] | 90 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 117.543, 42.530, 97.663 |
| Unit cell angles | 90.00, 107.63, 90.00 |
Refinement procedure
| Resolution | 93.080 - 1.960 |
| R-factor | 0.21915 |
| Rwork | 0.218 |
| R-free | 0.26752 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3zy2 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.432 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.010 | 2.060 |
| High resolution limit [Å] | 1.960 | 1.960 |
| Rmerge | 0.110 | 0.480 |
| Number of reflections | 42027 | |
| <I/σ(I)> | 12.19 | 12.19 |
| Completeness [%] | 98.0 | 94.7 |
| Redundancy | 7.06 | 5.01 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
