3ZVN
The structural basis for substrate recognition by mammalian polynucleotide kinase 3' phosphatase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-02-14 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 69.270, 72.990, 114.440 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.495 - 2.150 |
| R-factor | 0.2082 |
| Rwork | 0.205 |
| R-free | 0.26890 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1yj5 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.298 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.500 | 2.270 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.110 | 0.660 |
| Number of reflections | 28753 | |
| <I/σ(I)> | 8.5 | 2.3 |
| Completeness [%] | 89.2 | 92.7 |
| Redundancy | 4.3 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.1 M BIS TRIS PH 5.5, 0.1 M AMMONIUM ACETATE, 17 % (W/V) PEG 10000 |
