3ZVM
The structural basis for substrate recognition by mammalian polynucleotide kinase 3' phosphatase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR-H |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-02-15 |
| Detector | BRUKER |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.817, 75.243, 135.199 |
| Unit cell angles | 90.00, 96.30, 90.00 |
Refinement procedure
| Resolution | 38.490 - 1.997 |
| R-factor | 0.1545 |
| Rwork | 0.152 |
| R-free | 0.20060 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1yj5 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.099 |
| Data reduction software | PROTEUM2 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.500 | 2.040 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.120 | 0.310 |
| Number of reflections | 60655 | |
| <I/σ(I)> | 9.32 | 3.5 |
| Completeness [%] | 99.8 | 98.5 |
| Redundancy | 5.12 | 3.75 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.1 M HEPES PH 7.0, 18% (W/V) PEG 12000 |
