3ZUV
Crystal structure of a designed selected Ankyrin Repeat protein in complex with the phosphorylated MAP kinase ERK2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-05-02 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 113.670, 150.449, 104.970 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 77.120 - 2.720 |
| R-factor | 0.17837 |
| Rwork | 0.176 |
| R-free | 0.22975 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 2ERK 1mj0 |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.939 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 8.120 | 2.730 |
| High resolution limit [Å] | 2.720 | 2.720 |
| Rmerge | 0.050 | 0.410 |
| Number of reflections | 84304 | |
| <I/σ(I)> | 16.43 | 2.41 |
| Completeness [%] | 89.8 | 74.3 |
| Redundancy | 2.2 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 100 MM TRIS-HOAC (PH 7.5), 2 M SODIUM FORMATE |
