3ZRF

pVHL54-213-EloB-EloC complex_apo

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	CCD
Collection date	2010-05-27
Detector	ADSC QUANTUM 315r
Spacegroup name	P 41 2 2
Unit cell lengths	93.076, 93.076, 364.585
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.540 - 2.800
R-factor	0.22755
Rwork	0.223
R-free	0.32048
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1vcb
RMSD bond length	0.022
RMSD bond angle	2.262
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.960
High resolution limit [Å]	2.800	2.800
Rmerge	0.110	0.460
Number of reflections	74099
<I/σ(I)>	12.3	2.87
Completeness [%]	98.2	92.9
Redundancy	5	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				0.1 M NA CACODYLATE PH 5.8, 0.2 M MG ACETATE, 15% PEG8000, 5MM DTT.