3ZRC
pVHL54-213-EloB-EloC complex (4R)-4-HYDROXY-1-[(3-METHYLISOXAZOL-5-YL)ACETYL]-N-[4-(1,3-OXAZOL-5-YL)BENZYL]-L-PROLINAMIDE bound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-02-24 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 41 2 2 |
| Unit cell lengths | 93.741, 93.741, 363.184 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.920 - 2.900 |
| R-factor | 0.24772 |
| Rwork | 0.242 |
| R-free | 0.35042 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3RZF (APO STRUCTURE V54BC) |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.921 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.390 | 3.060 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.120 | 0.510 |
| Number of reflections | 34397 | |
| <I/σ(I)> | 9.6 | 2.5 |
| Completeness [%] | 93.9 | 91.9 |
| Redundancy | 4.8 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.1.M NA CITRATE PH 5.6, 0.2 M MG ACETATE, 15% PEG 8000, 50.MM DTT. |
