3ZRC

pVHL54-213-EloB-EloC complex (4R)-4-HYDROXY-1-[(3-METHYLISOXAZOL-5-YL)ACETYL]-N-[4-(1,3-OXAZOL-5-YL)BENZYL]-L-PROLINAMIDE bound

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2011-02-24
Detector	ADSC QUANTUM 315r
Spacegroup name	P 41 2 2
Unit cell lengths	93.741, 93.741, 363.184
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.920 - 2.900
R-factor	0.24772
Rwork	0.242
R-free	0.35042
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3RZF (APO STRUCTURE V54BC)
RMSD bond length	0.015
RMSD bond angle	1.921
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.390	3.060
High resolution limit [Å]	2.900	2.900
Rmerge	0.120	0.510
Number of reflections	34397
<I/σ(I)>	9.6	2.5
Completeness [%]	93.9	91.9
Redundancy	4.8	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				0.1.M NA CITRATE PH 5.6, 0.2 M MG ACETATE, 15% PEG 8000, 50.MM DTT.