3ZPQ
Thermostabilised turkey beta1 adrenergic receptor with 4-(piperazin-1- yl)-1H-indole bound (compound 19)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-02-02 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 89.852, 61.441, 100.784 |
| Unit cell angles | 90.00, 108.89, 90.00 |
Refinement procedure
| Resolution | 95.360 - 2.800 |
| R-factor | 0.22298 |
| Rwork | 0.220 |
| R-free | 0.27370 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4amj |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.297 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 61.400 | 2.950 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.180 | 0.650 |
| Number of reflections | 23841 | |
| <I/σ(I)> | 6.8 | 1.9 |
| Completeness [%] | 92.5 | 90.9 |
| Redundancy | 3.3 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 9 | 277 | 0.1M BICINE PH9.0, 24% PEG600, 4C |
