3ZLM
Fic protein from Neisseria meningitidis mutant E186G in complex with AMPPNP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-05-11 |
| Detector | DECTRIS PILATUS-2M-F |
| Spacegroup name | P 64 2 2 |
| Unit cell lengths | 149.080, 149.080, 76.440 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 2.000 |
| R-factor | 0.179 |
| Rwork | 0.178 |
| R-free | 0.19588 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2g03 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.159 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.320 | 2.100 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.060 | 0.730 |
| Number of reflections | 34254 | |
| <I/σ(I)> | 30.91 | 5.7 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 21.4 | 22.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 9 | 4M POTASSIUM FORMATE, 0.1M BIS-TRIS PROPANE PH 9.0, 2% W/V PEG MME 2000 |
