3ZLI
Crystal structure of JmjC domain of human histone demethylase UTY
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-12-15 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 90.585, 110.249, 118.242 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.010 - 1.800 |
| R-factor | 0.17741 |
| Rwork | 0.176 |
| R-free | 0.20435 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3avs |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.533 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.960 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.072 | 0.900 |
| Number of reflections | 110147 | |
| <I/σ(I)> | 13.4 | 2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.7 | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.2M TRIMETHYLAMINE N-OXIDE; 0.1M HEPES PH 7.5; 10%(W/V) PEG 3350 |
