3ZKJ
Crystal Structure of Ankyrin Repeat and Socs Box-Containing Protein 9 (Asb9) in Complex with Elonginb and Elonginc
Replaces: 2XAIExperimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Collection date | 2010-02-27 |
| Spacegroup name | P 21 2 21 |
| Unit cell lengths | 102.860, 110.740, 113.410 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.470 - 2.580 |
| R-factor | 0.2289 |
| Rwork | 0.227 |
| R-free | 0.26507 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.520 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.470 | 2.720 |
| High resolution limit [Å] | 2.580 | 2.580 |
| Rmerge | 0.100 | 0.770 |
| Number of reflections | 41297 | |
| <I/σ(I)> | 10.7 | 2.1 |
| Completeness [%] | 99.7 | 99.7 |
| Redundancy | 6.7 | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.2M NA2SO4, 0.1M BTPROP 6.5, 20% PEG 3350, 10% ETGLY |
