3ZKF

Structure of LC8 in complex with Nek9 phosphopeptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	100
Detector technology	PIXEL
Detector	DECTRIS PILATUS 6M
Spacegroup name	P 63
Unit cell lengths	154.868, 154.868, 47.729
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.967 - 2.600
R-factor	0.2222
Rwork	0.220
R-free	0.26860
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.196
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.730	2.630
High resolution limit [Å]	2.490	2.490
Rmerge	0.100	1.000
Number of reflections	22982
<I/σ(I)>	7.6	1.5
Completeness [%]	99.2	95.6
Redundancy	3.4	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1