3ZHA
Molecular basis for the action of the collagen-specific chaperone Hsp47 SERPINH1 and its structure-specific client recognition.
Replaces: 4AWRExperimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-06-06 |
| Detector | DECTRIS PILATUS |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 102.709, 104.905, 171.840 |
| Unit cell angles | 90.00, 103.71, 90.00 |
Refinement procedure
| Resolution | 49.160 - 2.550 |
| R-factor | 0.1956 |
| Rwork | 0.195 |
| R-free | 0.21760 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4au2 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.140 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.620 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Rmerge | 0.100 | 1.080 |
| Number of reflections | 113422 | |
| <I/σ(I)> | 9.1 | 0.94 |
| Completeness [%] | 98.1 | 94.3 |
| Redundancy | 2.3 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | pH 7 |
