3ZF5
Phage dUTPases control transfer of virulence genes by a proto-oncogenic G protein-like mechanism. (Staphylococcus bacteriophage 80alpha dUTPase Y84F mutant with dUpNHpp).
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Temperature [K] | 110 |
| Spacegroup name | P 21 3 |
| Unit cell lengths | 88.240, 88.240, 88.240 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 14.710 - 3.200 |
| R-factor | 0.22421 |
| Rwork | 0.222 |
| R-free | 0.27663 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3zez |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.858 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 14.900 | 3.160 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.170 | 0.350 |
| Number of reflections | 4719 | |
| <I/σ(I)> | 0.35 | 2.3 |
| Completeness [%] | 98.5 | 100 |
| Redundancy | 4.4 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 2-8% TERT-BUTANOL, 0.1M TRIS (PH 8.5), 30-50% MPD PR PEG400. |
