3ZEZ
Phage dUTPases control transfer of virulence genes by a proto- oncogenic G protein-like mechanism.(Staphylococcus bacteriophage 80alpha dUTPase with dUPNHPP).
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 21 3 |
| Unit cell lengths | 88.657, 88.657, 88.657 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.650 - 2.800 |
| R-factor | 0.21999 |
| Rwork | 0.216 |
| R-free | 0.26362 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2hqu |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.021 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.330 | 2.950 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.070 | 0.750 |
| Number of reflections | 5949 | |
| <I/σ(I)> | 23.9 | 4.7 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 6 | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 2-6% TERT-BUTANOL, 0.1M TRIS (PH 8.5), 30-50% MPD OR PEG400 |
