3ZEV

Structure of Thermostable Agonist-bound Neurotensin Receptor 1 Mutant without Lysozyme Fusion

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Detector	DECTRIS PILATUS 6M
Spacegroup name	P 21 21 21
Unit cell lengths	58.550, 90.180, 209.360
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.935 - 3.000
R-factor	0.2436
Rwork	0.242
R-free	0.27920
Structure solution method	OTHER
Starting model (for MR)	NONE
RMSD bond length	0.003
RMSD bond angle	0.735
Data reduction software	XDS
Data scaling software	XSCALE
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	3.000
Rmerge	0.010
Number of reflections	22942
<I/σ(I)>	8.68
Completeness [%]	99.8
Redundancy	6.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		9.4		1.28% (W/V) NONYL-GLUCOSIDE, 0.5% (W/V) DECYL-GLUCOSIDE, 0.01% (W/V) DODECYL-GLUCOSIDE, 0.1% (W/V) CHOLESTERYLHEMISUCCINATE, 10MM HEPES PH 8, 1.15 MM NACL, 2 MM DTT, 100 NM NTI, 26% (V/V) PEG 600, 50 MM GLYCINE PH 9.4