3ZDF
Structure of GAPDH with CP12 peptide from Thermosynechococcus elongatus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-03-03 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 141.450, 141.450, 77.390 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 63.260 - 2.340 |
| R-factor | 0.17539 |
| Rwork | 0.173 |
| R-free | 0.21464 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3zcx |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.746 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 63.260 | 2.400 |
| High resolution limit [Å] | 2.340 | 2.340 |
| Rmerge | 0.140 | 0.650 |
| Number of reflections | 33680 | |
| <I/σ(I)> | 5.38 | 1.18 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 6.61 | 6.53 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.1 M BICINE PH 9, 10 % W/V PEG 20,000/ 2% V/V DIOXANE |
