3ZDB
Structure of E. coli ExoIX in complex with the palindromic 5ov4 DNA oligonucleotide, di-magnesium and potassium
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-03-04 |
Detector | ADSC QUANTUM 315r |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 66.238, 152.302, 34.522 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.670 - 1.470 |
R-factor | 0.20425 |
Rwork | 0.203 |
R-free | 0.23054 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | EXOIX FLAP1 FRAGMENT COMPLEX STRUCTURE |
RMSD bond length | 0.013 |
RMSD bond angle | 1.440 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 33.670 | 1.550 |
High resolution limit [Å] | 1.470 | 1.470 |
Rmerge | 0.050 | 0.240 |
Number of reflections | 60541 | |
<I/σ(I)> | 15.2 | 4.7 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 4.1 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.5 | 0.2 M MAGNESIUM ACETATE, 15% (W/V) PEG-3350, pH 6.5 |