3ZD7

Snapshot 3 of RIG-I scanning on RNA duplex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	287
Detector technology	CCD
Detector	ADSC QUANTUM 315
Spacegroup name	P 21 21 21
Unit cell lengths	48.252, 75.996, 207.279
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.000 - 2.500
R-factor	0.23205
Rwork	0.229
R-free	0.28791
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ykg
RMSD bond length	0.012
RMSD bond angle	1.515
Phasing software	PHASER
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.300	2.640
High resolution limit [Å]	2.500	2.500
Rmerge	0.060	0.570
Number of reflections	26729
<I/σ(I)>	11.4	1.9
Completeness [%]	98.7	99.4
Redundancy	3.4	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		9		pH 9