3YGS

APAF-1 CARD IN COMPLEX WITH PRODOMAIN OF PROCASPASE-9

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Spacegroup name	P 62 2 2
Unit cell lengths	92.500, 92.500, 136.800
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	10.000 - 2.500
R-factor	0.224 *
Rwork	0.224
R-free	0.29900
Structure solution method	MIR
RMSD bond length	0.009
RMSD bond angle	1.447
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	X-PLOR (3.8)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	99.000	2.590
High resolution limit [Å]	2.500	2.500
Rmerge	0.056	0.177
Total number of observations	134080 *
Number of reflections	12452 *
Completeness [%]	98.6	93.2
Redundancy	11

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5	4 *	pH 7.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	HEPES	100 (mM)
2	1	reservoir	potassium sodium dihydrogen phosphate	1.75 (M)
3	1	reservoir	dithiothreitol	10 (mM)