3X42
Crystal structure of copper amine oxidase from Arthrobacter globiformis in the presence of sodium bromide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL38B1 |
Synchrotron site | SPring-8 |
Beamline | BL38B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-02-09 |
Detector | ADSC QUANTUM 210r |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 192.684, 63.508, 158.099 |
Unit cell angles | 90.00, 117.55, 90.00 |
Refinement procedure
Resolution | 24.738 - 1.875 |
R-factor | 0.1595 |
Rwork | 0.158 |
R-free | 0.19000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1iu7 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.083 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Refinement software | PHENIX (1.8_1069) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 | 1.960 |
High resolution limit [Å] | 1.875 | 1.890 |
Rmerge | 0.076 | 0.471 |
Number of reflections | 267101 | |
<I/σ(I)> | 12.5 | |
Completeness [%] | 99.3 | 98.7 |
Redundancy | 3.5 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.8 | 289 | 1.05M potassium-sodium tartrate, 25mM HEPES (pH6.8), MICRODIALYSIS, temperature 289K |