3X3Z

Copper amine oxidase from Arthrobacter globiformis: Aminoresorcinol form produced by anaerobic reduction with ethylamine hydrochloride

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL38B1
Synchrotron site	SPring-8
Beamline	BL38B1
Temperature [K]	100
Detector technology	CCD
Collection date	2009-07-10
Detector	ADSC QUANTUM 210r
Wavelength(s)	0.9
Spacegroup name	C 1 2 1
Unit cell lengths	192.548, 62.729, 157.647
Unit cell angles	90.00, 117.62, 90.00

Refinement procedure

Resolution	22.281 - 1.510
R-factor	0.1615
Rwork	0.161
R-free	0.17830
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1iu7
RMSD bond length	0.011
RMSD bond angle	1.153
Data reduction software	MOSFLM
Data scaling software	SCALA (3.2.25)
Refinement software	PHENIX (1.8_1069)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	38.519	38.519	1.590
High resolution limit [Å]	1.500	4.780	1.510
Rmerge	0.092	0.056	0.404
Rpim		0.024	0.163
Total number of observations		54557	260450
Number of reflections	260515
<I/σ(I)>	17.3	39.8	4
Completeness [%]	99.8	96.8	99.8
Redundancy	7.1	6.6	6.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICRODIALYSIS	6.8	289	1.05M potassium-sodium tartrate, 25mM HEPES (pH6.8), MICRODIALYSIS, temperature 289K