3WZU

THE STRUCTURE OF MAP2K7 IN COMPLEX WITH 5Z-7-oxozeaenol

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	CCD
Collection date	2014-02-08
Detector	ADSC QUANTUM 4r
Spacegroup name	P 61 2 2
Unit cell lengths	71.395, 71.395, 268.817
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	36.280 - 3.010
R-factor	0.23959
Rwork	0.236
R-free	0.32182
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2dyl
RMSD bond length	0.011
RMSD bond angle	1.839
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC (5.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.300	36.300
High resolution limit [Å]	3.000	30.100
Number of reflections	7998
Completeness [%]	90.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	277	25W/V PEG3350, 0.2M SODIUM CITRATE, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K