3WX1

Mouse Cereblon thalidomide binding domain, selenomethionine derivative

Experimental procedure

Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	CCD
Collection date	2011-01-26
Detector	RAYONIX MX-225
Wavelength(s)	0.97911, 0.99513, 0.97941
Spacegroup name	I 21 3
Unit cell lengths	118.112, 118.112, 118.112
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.100 - 1.930
R-factor	0.19057
Rwork	0.189
R-free	0.21224
Structure solution method	MAD
RMSD bond length	0.005
RMSD bond angle	0.993
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHARP
Refinement software	REFMAC (5.8.0049)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.930
Number of reflections	20774
Completeness [%]	100.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	5.7	277	0.1M sodium acetate (pH 5.7), 0.2M sodium sulphate, 12% PEG3350, 30% glycerol, VAPOR DIFFUSION, temperature 277K