3WV6

Crystal Structure of a protease-resistant mutant form of human galectin-9

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NE3A
Synchrotron site	Photon Factory
Beamline	AR-NE3A
Temperature [K]	100
Detector technology	CCD
Collection date	2012-10-29
Detector	ADSC QUANTUM 270
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	58.340, 94.870, 64.810
Unit cell angles	90.00, 113.87, 90.00

Refinement procedure

Resolution	27.850 - 1.950
R-factor	0.192
Rwork	0.188
R-free	0.22200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2eak 2yro
RMSD bond length	0.005
RMSD bond angle	1.400
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.850	1.980
High resolution limit [Å]	1.950	1.950
Rmerge	0.099	0.286
Number of reflections	46506
<I/σ(I)>	13.5	3.99
Completeness [%]	98.9	100
Redundancy	3.8	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	0.2M L-proline, 4%(w/v) PEG3350, 0.1M HEPES, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K