3WV4
Crystal structure of VinN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-10-31 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9782 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 81.190, 109.820, 201.460 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.370 - 2.150 |
| R-factor | 0.2039 |
| Rwork | 0.202 |
| R-free | 0.24490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3au9 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.710 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.370 | 2.210 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Number of reflections | 44155 | |
| Completeness [%] | 89.5 | 83.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 278 | 0.1M Tris-HCl, 0.2M sodium acetate, 30% polyethylene glycol 4000, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 278K |
