3WPC
Crystal structure of horse TLR9 in complex with agonistic DNA1668_12mer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-04-21 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 68.762, 81.624, 81.519 |
| Unit cell angles | 87.50, 83.50, 85.98 |
Refinement procedure
| Resolution | 47.490 - 1.600 |
| R-factor | 0.18575 |
| Rwork | 0.184 |
| R-free | 0.22022 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.683 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.600 |
| Number of reflections | 211265 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.8 | 293 | 1% PEG 4000, 1% 2-propanol, 0.2M Na acetate, 0.1M Na citrate pH5.8, 5% DMSO, 1/10 volume of Silver Bullet A6, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
