3WO2
Crystal structure of human interleukin-18
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-10-22 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 68.146, 79.511, 73.460 |
| Unit cell angles | 90.00, 100.97, 90.00 |
Refinement procedure
| Resolution | 34.820 - 2.330 |
| R-factor | 0.2206 |
| Rwork | 0.218 |
| R-free | 0.27140 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3f62 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.380 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.960 | 2.460 |
| High resolution limit [Å] | 2.330 | 2.330 |
| Rmerge | 0.049 | 0.396 |
| Number of reflections | 32990 | |
| <I/σ(I)> | 20.5 | 3.6 |
| Completeness [%] | 97.8 | 97.1 |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | 100mM Bis-Tris-HCl, 2.5M ammonium sulfate, 0.2%(w/v) CHAPS, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277.0K |
