3WMK
Crystal structure of Hen egg-white lysozyme in pH 4.5 Sodium Acetatewith 1M NaCl at 277K
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 6C1 |
| Synchrotron site | PAL/PLS |
| Beamline | 6C1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-07-23 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.97949 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 78.665, 78.665, 36.922 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.810 - 1.460 |
| R-factor | 0.176 |
| Rwork | 0.173 |
| R-free | 0.23476 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.021 |
| RMSD bond angle | 2.161 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASES |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 |
| High resolution limit [Å] | 1.460 | 1.460 |
| Rmerge | 0.150 | 0.150 |
| Number of reflections | 228340 | |
| <I/σ(I)> | 39.755 | 2.28 |
| Completeness [%] | 97.7 | 100 |
| Redundancy | 11.3 | 11.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 277 | 80mg/ml hen egg-white lysozyme, 100mM Sodium Acetate, 1M NaCl , pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
