3WHO
X-ray-Crystallographic Structure of an RNase Po1 Exhibiting Anti-tumor Activity
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-06-27 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.9800 |
| Spacegroup name | P 31 |
| Unit cell lengths | 75.557, 75.557, 34.803 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.730 - 1.850 |
| R-factor | 0.205 |
| Rwork | 0.203 |
| R-free | 0.24960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2bu4 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.962 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.780 | 1.950 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.157 | 0.627 |
| Number of reflections | 18553 | 1561 |
| <I/σ(I)> | 10.1 | 2.5 |
| Completeness [%] | 99.6 | 84.7 |
| Redundancy | 7.7 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 4M sodium formate, 0.1M Bis-TRIS propane, 10% PEG400, 0.75M cesium chloride, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
