3WDZ
Crystal Structure of Keap1 in Complex with phosphorylated p62
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-06-20 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 75.149, 75.149, 113.008 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 42.670 - 2.600 |
| R-factor | 0.19367 |
| Rwork | 0.191 |
| R-free | 0.25491 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1x2j |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.831 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.640 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.081 | 0.376 |
| Number of reflections | 11646 | |
| <I/σ(I)> | 14.5 | 6.51 |
| Completeness [%] | 99.8 | 98.8 |
| Redundancy | 5.2 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.6 | 297 | 100mM Tris-HCl (pH 8.8), 0.2M Sodium Nitrate, 20% w/v PEG 3350, VAPOR DIFFUSION, SITTING DROP, temperature 297K |
