3WAJ
Crystal structure of the Archaeoglobus fulgidus oligosaccharyltransferase (O29867_ARCFU) complex with Zn and sulfate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44XU |
Synchrotron site | SPring-8 |
Beamline | BL44XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-06-10 |
Detector | RAYONIX MX325HE |
Wavelength(s) | 0.9000 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 234.195, 108.961, 56.069 |
Unit cell angles | 90.00, 96.08, 90.00 |
Refinement procedure
Resolution | 38.813 - 2.501 |
R-factor | 0.1842 |
Rwork | 0.182 |
R-free | 0.21570 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3wai |
RMSD bond length | 0.008 |
RMSD bond angle | 1.150 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.813 | 2.540 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.049 | 0.512 |
Number of reflections | 47863 | |
<I/σ(I)> | 22.9 | 2.3 |
Completeness [%] | 98.5 | 98.6 |
Redundancy | 3.8 | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | 0.2M zinc sulfate, 0.1M sodium acetate buffer, 15% PEG 4000, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |