3W81
Human alpha-l-iduronidase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
Synchrotron site | Photon Factory |
Beamline | AR-NW12A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-10-12 |
Detector | ADSC QUANTUM 210r |
Wavelength(s) | 1.0000 |
Spacegroup name | H 3 |
Unit cell lengths | 259.225, 259.225, 71.835 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 36.560 - 2.300 |
R-factor | 0.1711 |
Rwork | 0.169 |
R-free | 0.20990 |
Structure solution method | SIRAS |
RMSD bond length | 0.010 |
RMSD bond angle | 1.579 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHARP |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.370 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.102 | 0.468 |
Number of reflections | 78102 | |
<I/σ(I)> | 13.2 | 3 |
Completeness [%] | 97.4 | 80.6 |
Redundancy | 6.6 | 4.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 288 | 18%(w/v) PEG 3350, 0.18M K/Na tartrate, 3%(w/v) PEG MME 5000, 0.02M ammonium sulfate, 0.01M MES-Na, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 288K |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 288 | 18%(w/v) PEG 3350, 0.18M K/Na tartrate, 3%(w/v) PEG MME 5000, 0.02M ammonium sulfate, 0.01M MES-Na, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 288K |