3W3S
Crystal structure of A. aeolicus tRNASec in complex with M. kandleri SerRS
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 90 |
Detector technology | CCD |
Collection date | 2009-11-27 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97848 |
Spacegroup name | I 4 3 2 |
Unit cell lengths | 272.817, 272.817, 272.817 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.823 - 3.095 |
R-factor | 0.2064 |
Rwork | 0.204 |
R-free | 0.26030 |
Structure solution method | MAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.253 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHARP |
Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.210 |
High resolution limit [Å] | 3.095 | 3.100 |
Rmerge | 0.094 | 0.847 |
Number of reflections | 31714 | |
<I/σ(I)> | 28.4 | 3.49 |
Completeness [%] | 99.9 | 100 |
Redundancy | 15 | 14.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 293 | 100mM trisodium citrate-HCl, 1.3M ammonium sulfate, 210mM potassium sodium tartrate, 50mM imidazole, 400mM NaCl, 10mM MgCl2, 0.5mM 5'-O-[N-(L-seryl)sulfamoyl]adenosine, pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 293 | 100mM trisodium citrate-HCl, 1.3M ammonium sulfate, 210mM potassium sodium tartrate, 50mM imidazole, 400mM NaCl, 10mM MgCl2, 0.5mM 5'-O-[N-(L-seryl)sulfamoyl]adenosine, pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K |