3VXV
Crystal structure of methyl CpG Binding Domain of MBD4 in complex with the 5mCG/TG sequence
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2010-01-27 |
Detector | ADSC QUANTUM 210r |
Wavelength(s) | 1.000 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 89.074, 94.989, 54.738 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 23.747 - 2.000 |
R-factor | 0.1892 |
Rwork | 0.188 |
R-free | 0.22350 |
Structure solution method | SAD |
RMSD bond length | 0.018 |
RMSD bond angle | 2.071 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.031 | 0.365 |
Number of reflections | 16062 | |
<I/σ(I)> | 18.2 | 4.3 |
Completeness [%] | 99.7 | 100 |
Redundancy | 7.2 | 7.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.4 | 293 | pH 4.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
2 | VAPOR DIFFUSION, HANGING DROP | 4.4 | 293 | pH 4.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |