3VW6
Crystal structure of human apoptosis signal-regulating kinase 1 (ASK1) with imidazopyridine inhibitor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.3 |
| Synchrotron site | ALS |
| Beamline | 5.0.3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-08-27 |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 78.032, 78.032, 425.295 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 70.880 - 2.400 |
| R-factor | 0.21472 |
| Rwork | 0.213 |
| R-free | 0.25178 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.286 |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.400 |
| Number of reflections | 31268 |
| Completeness [%] | 98.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 277 | 10% PEG 6000, 1M lithium chloride, 0.1M HEPES, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 277.0K |
