3VUZ

Crystal structure of histone methyltransferase SET7/9 in complex with AAM-1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	100
Detector technology	CCD
Collection date	2010-10-16
Detector	ADSC QUANTUM 210
Spacegroup name	P 21 21 2
Unit cell lengths	101.807, 39.223, 67.045
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.190 - 2.500
R-factor	0.1864
Rwork	0.183
R-free	0.24660
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1n6a
RMSD bond length	0.007
RMSD bond angle	1.111
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7.3_928))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.590
High resolution limit [Å]	2.500	2.500
Number of reflections	9883
<I/σ(I)>	16	3.2
Completeness [%]	99.9	100
Redundancy	7.1	7.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	0.1M Tris-HCl pH8.3-8.5, 28-30% PEG 6000, VAPOR DIFFUSION, HANGING DROP, temperature 277K