3VRQ
Crystal structure of the tyrosine kinase binding domain of Cbl-c (PL mutant)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2008-06-18 |
| Detector | Bruker DIP-6040 |
| Wavelength(s) | 0.900 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 62.600, 66.211, 81.558 |
| Unit cell angles | 90.00, 106.02, 90.00 |
Refinement procedure
| Resolution | 37.980 - 2.390 |
| R-factor | 0.2176 |
| Rwork | 0.215 |
| R-free | 0.26380 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.468 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (refmac_5.5.0109) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.490 |
| High resolution limit [Å] | 2.390 | 5.170 | 2.400 |
| Rmerge | 0.062 | 0.023 | 0.373 |
| Number of reflections | 25297 | ||
| <I/σ(I)> | 22.777 | 46.682 | 4.006 |
| Completeness [%] | 100.0 | 99.8 | 100 |
| Redundancy | 3.8 | 3.7 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 288 | 22% PEG3350, 0.1M Magnesium Nitrate, 0.6M NDSB-201, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 288K |
