3VR8
Mitochondrial rhodoquinol-fumarate reductase from the parasitic nematode Ascaris suum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2004-10-05 |
| Detector | Bruker DIP-6040 |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 123.707, 129.090, 221.167 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.560 - 2.810 |
| R-factor | 0.23446 |
| Rwork | 0.231 |
| R-free | 0.29717 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1zoy |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.699 |
| Data reduction software | DENZO |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.850 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.117 | 0.517 |
| Number of reflections | 79622 | |
| <I/σ(I)> | 7.9 | 1.26 |
| Completeness [%] | 92.5 | 67.5 |
| Redundancy | 8.8 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICRODIALYSIS | 8.4 | 293 | 15% (w/v) PEG 3350, 100mM Tris-HCl pH 8.4, 200mM NaCl, 1mM sodium malonate, 0.06% (w/v) C12E8, 0.04% (w/v) C12M, MICRODIALYSIS, temperature 293K |
