3VHV
Mineralocorticoid receptor ligand-binding domain with non-steroidal antagonist
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.3 |
| Synchrotron site | ALS |
| Beamline | 5.0.3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-02-17 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 32 |
| Unit cell lengths | 62.612, 62.612, 75.504 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.000 - 1.350 |
| R-factor | 0.14247 |
| Rwork | 0.141 |
| R-free | 0.16735 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ab2 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.243 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.400 |
| High resolution limit [Å] | 1.350 | 1.350 |
| Number of reflections | 71863 | |
| <I/σ(I)> | 22.5 | 2.2 |
| Completeness [%] | 98.6 | 88.4 |
| Redundancy | 3.7 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 293 | 0.1M HEPES pH 7.4, 0.88M potassium/sodium tartrate, 5% ethylene glycol, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
