3VHT
Crystal structure of GFP-Wrnip1 UBZ domain fusion protein in complex with ubiquitin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-12-17 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 88.950, 143.692, 106.864 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.400 |
| R-factor | 0.1935 |
| Rwork | 0.191 |
| R-free | 0.23768 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ai5 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.820 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 75.630 | 2.440 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.105 | 0.534 |
| Number of reflections | 26943 | |
| <I/σ(I)> | 21.9 | 3.7 |
| Completeness [%] | 99.4 | 99.8 |
| Redundancy | 5.8 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 0.2M DL-Malic acid, 20% PEG 3350, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293.0K |
